Cistanche deserticola plus bone marrow mesenchymal stem cell transplantation for treating spinal cord injury in rats

doi:10.3969/j.issn.2095-4344.2014.41.014

Abstract

Abstract:

BACKGROUND: Studies have shown that bone marrow mesenchymal stem cells under certain conditions can differentiate into nerve cells. Bone marrow mesenchymal stem cell transplantation can rebuild nervous system function and improve functional disorders in patients. Glycosides of cistanche also have a protective effect against nerve cell injury. Their combination has been less reported.

OBJECTIVE: To observe the effectiveness of Cistanche deserticola and bone marrow mesenchymal stem cell transplantation on spinal cord injury in rats.

METHODS: Fifty adult Wistar rats with spinal cord injury were randomly divided into four group: a Cistanche deserticola group (intragastric administration of 20 mL/kg Cistanche deserticola concentrated solution per day), a cell transplantation group (10 μL of 1×10⁸/L bone marrow mesenchymal stem cell suspension), a combination group (10 μL of 1×10⁸/L bone marrow mesenchymal stem cell suspension+ intragastric administration of

20 mL/kg Cistanche deserticola concentrated solution per day) and a control group (intragastric administration of 20 mL/kg normal saline per day). The intragastric administration lasted for 30 days in each group.

RESULTS AND CONCLUSION: After 30 days of treatment, the expression of Nestin was significantly higher in the combination group than the other groups. After 12 weeks, Basso, Beattie, and Bresnahan scores was significantly higher in the combination group than the other groups (P < 0.05); somatosensory and motor evoked potential latencies were also improve significantly in the combination group compared with the other groups (P < 0.05). These findings indicate that oral administration of Cistanche deserticola combined with bone marrow mesenchymal stem cells can significantly improve the motor and neurophysiological function of spinal cord injury rats. Cistanche deserticola can improve the survival of transplanted bone marrow mesenchymal stem cells in rats with spinal cord injury.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: bone marrow, mesenchymal stem cells, cistanche, spinal cord injuries, rats, Wistar

CLC Number:

R394.2

Lan Jing, Yan Jin-yu, Xia Run-fu, Li Jian-feng. Cistanche deserticola plus bone marrow mesenchymal stem cell transplantation for treating spinal cord injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(41): 6639-6644.

Figures/Tables 1

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